The Irish FA confirmed on Monday that Daryl Murphy was having a scan to determine the extent of his calf problem.
Following the results of the scan, the Newcastle striker has now been ruled out of Friday’s match against Wales.
Daryl Murphy will now return to Tyneside to be assessed.
The former Ipswich star completes a trio of Newcastle players to be ruled out for Ireland, following on from Ciaran Clark and Rob Elliot who are both reported to have knee injuries.
Daryl Murphy was preferred to both Dwight Gayle and Aleksandar Mitrovic for the starting eleven at Birmingham on Saturday before making way just after an hour for United’s number 9 to have a go.
The former Ipswich striker came closest to breaking the deadlock, his perfectly placed header only been denied by a superb fingertip save by Tomasz Kuszczak.
Monday morning saw Newcastle United confirm that Grant Hanley had also been forced to drop out of the Scotland squad – in his case it is an ankle injury.
Daryl Murphy has been in great form and has scored against both Huddersfield and Fulham this month, to go with his goals against Rotherham and Brentford.
Despite only starting five Championship matches (plus five as a sub) this season, Murphy’s four goals have shown the value of the 34 year old who had scored 50 league goals over the course of the past three seasons.
Worryingly, it was a calf injury that kept Daryl Murphy on the sidelines earlier this campaign and it was also a calf problem that ruled him out at Ipswich for a period of time last season.